FastQCFastQC Report
Sun 14 Apr 2019
SRR6576610_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576610_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135445
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3160.23330503156262689No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2420.1786703089815054No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2230.1646424748052715No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2080.1535678688766658No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2070.15282956181475876No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1950.1439698770718742No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1630.12034405109084868No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1600.11812912990512754No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC1530.11296098047177822No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1520.11222267340987116No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1450.10705452397652183No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG1400.10336298866698661No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG1400.10336298866698661No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1380.10188637454317251No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCTAT555.14673E-542.8827741
CTATTCC853.9233782E-738.7032974
ATATAAC651.4074687E-436.1514323
CCTATTC959.37398E-734.6292653
CCTTATG550.002709477434.179542
TCTTGCG702.1757315E-433.5691872
CGCCGGT600.004149504531.3312427
CTGTGCG600.004149504531.3312429
CATGGGT1951.8189894E-1128.9211464
GACAAAT650.00613727628.9211447
GCCGGTC650.00613727628.9211448
TCCTATT1354.586691E-727.849992
GTACAGC856.642156E-427.7476791
TACATGG10650.026.918392
CTTATGT700.00881188626.8553493
TATAACC700.00881188626.8553494
GTACATG10950.026.7087251
GTCTTGC1257.734216E-626.415791
ACATGGG10600.025.7152633
GGGTACC1659.197174E-825.6346517
Produced by FastQC (version 0.11.2)