FastQCFastQC Report
Sun 14 Apr 2019
SRR6576611_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576611_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125189
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG3480.27797969470161116No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2280.18212462756312456No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2090.16694757526619752No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1810.14458139293388397No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1740.13898984735080558No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA1560.12461158728003258No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG1520.12141641837541638No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA1430.11422728834002988No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC1380.11023332720925959No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC1320.10544057385233527No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA1310.10464178162618121No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1290.10304419717387311No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC1280.10224540494771905No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG1270.10144661272156501No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT1260.10064782049541093No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCGAT250.005224204656.40263794
ATACTGA250.005232477656.38011
TACAGCG250.005232477656.38015
ATGGGAG1350.045.2432945
GCAAACG450.001012247941.7630421
GACTGTA450.001012247941.7630427
GAATAAG500.001699181637.5867351
TACAGTA500.001699181637.5867357
ATTATAC550.002712498434.169763
GTACATG9550.033.4541631
TACATGG9450.033.3109972
ACATGGG9500.032.6411133
ATACGCT1354.5934758E-727.8420288
CATGGGG3800.027.2009264
ATGGAAG700.00882167826.847677
TACGCTA1406.3118387E-726.847679
ATATACG1406.3118387E-726.847676
CTCAGAC700.00882167826.847673
TGGGTAC1257.971299E-626.3107176
GGGTACC1257.971299E-626.3107177
Produced by FastQC (version 0.11.2)