FastQCFastQC Report
Sun 14 Apr 2019
SRR6576612_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576612_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences375514
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9100.24233450683596353No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6110.16271031173271835No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5680.1512593405305794No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5460.1454007041015781No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4980.13261822462012068No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4650.12383026997661872No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4410.11743903023589No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4280.11397710870966196No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4270.11371080705379825No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4170.1110477904951613No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4090.10891737724825173No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3970.10572175737788739No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3870.10305874081925043No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3820.10172723253993193No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG654.737558E-850.688841
GTATCAA9900.037.559481
GTACATG20700.032.970491
TACATGG21300.031.9948042
ATGGGTA4150.031.710355
ACATGGG20750.031.4838473
GTCTAGG600.004128036631.3788051
CCTATAG953.7460224E-529.6837483
AACGCAG12400.028.8060026
ACTAGGC1005.314689E-528.199563
CAACGCA12950.027.9455095
TAGGCAT856.808105E-427.6466275
ATCAACG12800.027.5386333
TCAACGC13100.027.266754
GATATAA1406.2692015E-726.896121
ATACAGC1258.006089E-626.319596
GGTACCT3800.025.973288
ACGCAGA13650.025.8237727
TCTAGAC1101.01665624E-425.6359633
ATATAGG1101.01665624E-425.6359633
Produced by FastQC (version 0.11.2)