FastQCFastQC Report
Sun 14 Apr 2019
SRR6576613_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576613_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338484
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10600.31316103567672327No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6230.18405596719490433No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5490.16219378168539725No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5350.15805769253494995No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5110.15096725399132602No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4600.1359000720861252No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4520.1335365925715839No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3990.11787854078774772No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3880.11462875645525342No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3740.11049266730480613No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3690.10901549260821783No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3670.10842462272958248No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3620.10694744803299418No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC3560.10517483839708819No Hit
CCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGG3550.10487940345777053No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3490.10310679382186455No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3470.10251592394322923No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA3460.10222048900391156No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA3460.10222048900391156No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3390.10015244442868791No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAA250.005239339556.382365
TATCCCG405.679761E-446.9853025
CATGGGT5550.037.2496074
GTATTAT801.1528651E-535.2650221
GTACTAT801.1528651E-535.2650221
ATGGGTA4150.035.0974545
GTAATAG600.004148207631.346691
TACTATC600.004148207631.346692
TATAGGT600.004163240531.3235344
GTATCAA14250.029.6968631
GTACATG26100.029.3650021
ACATGGG25900.029.025673
TACATGG26300.028.9629082
GTCAACG650.006135326328.9354041
TACTATA856.789142E-427.6588422
GTACACC856.818542E-427.6384123
GGGTACC4250.027.638417
ATGGGAG3800.027.2020175
GTGTAGA1406.322425E-726.868591
TGGGTAC4550.026.8487436
Produced by FastQC (version 0.11.2)