FastQCFastQC Report
Sun 14 Apr 2019
SRR6576613_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576613_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences338484
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9860.2912988501672162No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5730.16928422022902118No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5420.16012573711017358No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5300.15658051783836163No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4680.1382635516006665No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4210.12437810945273632No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4210.12437810945273632No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4080.12053745524160671No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4050.11965115042365371No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4030.11906028054501838No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3840.11344701669798278No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3670.10842462272958248No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3660.10812918779026483No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3620.10694744803299418No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA3510.10369766370049986No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3490.10310679382186455No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3410.10074331430732324No Hit
AGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTC3410.10074331430732324No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATAG652.780127E-643.382235
TATCCGT450.001013214941.775484
GTGTAAT1003.2694516E-837.6591381
GTACATG21950.033.2412641
TACATGG22000.032.898192
ACATGGG21950.032.7590183
GTATAGA600.004125011631.3826181
GTATCAA11300.031.2437531
ATGGGTA3550.029.125165
GTCTTAA1154.1339936E-628.6536921
CATGGGG9450.027.850324
AACGCAG12750.027.6414556
ATGGGAT1904.456524E-1027.2090285
TATAGAT1057.409146E-526.8556672
CATGGGT5250.026.8556654
ATATCCG700.00882974126.8556653
TACTAAA700.00882974126.8556652
ATGGGAG3350.026.6552495
TAGTACT909.506811E-426.1096744
CAACGCA13550.026.0133295
Produced by FastQC (version 0.11.2)