FastQCFastQC Report
Sun 14 Apr 2019
SRR6576615_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576615_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences361191
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8270.22896473057191347No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4200.11628196715865013No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4190.11600510533208191No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3750.10382318496308046No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3730.10326946130994404No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3700.10243887583023939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10100.042.3514021
GTACATG22450.040.6193661
TACATGG22950.039.7344132
ACATGGG23500.037.5991133
CATGGGT6700.034.3723224
ATCAACG12400.033.7330783
AACGCAG12200.033.5156026
TCAACGC12650.032.694884
CAACGCA12800.032.3117375
GGTACCT3500.032.227818
GGGTACC3650.032.191027
ATGGGTA3550.031.77395
GTACTAG753.268925E-431.3369331
CATGGGG7600.030.3019184
CATGGGA12100.030.2968084
ACGCAGA13700.029.8460127
GTATTAA953.742295E-529.6876221
ATGGGAG6150.029.0399675
TATCTCG650.006149363728.9223985
TGGGTAC4100.028.657866
Produced by FastQC (version 0.11.2)