FastQCFastQC Report
Sun 14 Apr 2019
SRR6576619_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576619_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264912
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4800.1811922449719152No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3240.12230476535604276No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3090.11664250770067042No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3040.11475508848221297No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2790.1053179923899257No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAC651.3921707E-436.234541
CCCTACA550.002713760434.1805152
GTATCAA9850.032.997341
ATAATAC1001.406157E-632.8987433
ACATGGG16600.032.2758753
TACATGG17400.031.6022422
GTACATG17600.031.0464131
CATGGGT3900.030.1270564
GTGTAAC804.7091712E-429.4405611
TATAGGT856.803668E-427.6460064
ATCAACG11650.027.432433
AACGCAG11850.026.9694356
CAACGCA12050.026.5218095
TCAACGC12050.026.5218094
CATGGGG5500.026.4898974
GTCTATG950.001285124124.7920531
ACGCAGA13200.024.2111977
TCTATGA1951.744229E-824.1016452
TATCAAC13400.023.8498382
GTACTTT7150.023.7171541
Produced by FastQC (version 0.11.2)