FastQCFastQC Report
Sun 14 Apr 2019
SRR6576620_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576620_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236487
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5850.24737089142320717No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2930.12389687382393112No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2740.11586260555548507No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2520.10655976861307387No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2510.10613691238841882No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2390.10106263769255816No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGT3250.036.144174
GTATCAA10300.035.1265141
AAGGCGT550.002716229834.172676
ACATGGG17100.034.072753
TACATGG17350.033.8526042
GTACATG17300.033.407241
ATGGGTT753.272874E-431.3249455
CTGTGCG753.272874E-431.3249459
CATGGGG6100.030.811424
AACGCAG11750.030.7917546
ATCAACG12450.029.060493
TCAACGC12600.028.714534
ACGCAGA12950.027.9384677
GGTACCT1853.255991E-1027.9384658
CAACGCA13100.027.6185595
TATCAAC13700.027.4379082
ATTAGGC350.008842397526.84995324-25
TCGCTTT350.008842397526.84995334-35
GCTTAGG700.0088336226.8499531
CGCAGAG13550.026.7013388
Produced by FastQC (version 0.11.2)