FastQCFastQC Report
Sun 14 Apr 2019
SRR6576620_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576620_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236487
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4960.20973668742890728No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3160.1336225669909974No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3130.1323539983170322No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2670.11290261198289969No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2520.10655976861307387No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2510.10613691238841882No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCTT550.002703934734.2042928
TGACCGC550.002715180634.175366
TATAAGT550.002715180634.175364
GTACATG17100.032.2131231
ACATGGG16400.032.0914963
TACATGG17200.031.6916852
GTATCAA11050.031.529171
ACTAGAT753.271362E-431.3274147
TATAGGG600.004158206731.3274142
CATGGGT3500.030.8798774
CATGGGG5950.030.0111354
ATGGGTA2550.029.4846215
GCTTATA650.00615009728.9176122
AACGCAG12350.028.5371176
GGTACCT2350.028.0184088
ATCAACG12800.027.533863
GTATTGG700.00874854426.9032691
TGGGTAC2450.026.8520686
TCAACGC13150.026.4436684
CAACGCA13200.026.3435085
Produced by FastQC (version 0.11.2)