FastQCFastQC Report
Sun 14 Apr 2019
SRR6576626_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576626_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129891
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2830.21787498748951045No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1880.14473674080575252No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1340.10316342163814275No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACGT156.889438E-493.996154
CAAACGC250.00522646456.397692
AAACGCA250.00522646456.397693
GATTAGT250.00522646456.397691
CCCGCTA250.00522646456.397693
TACATGG11300.039.5116542
ATGGGAG3450.039.5056235
GTACATG11350.038.9235151
ATGGGGC1101.7716957E-938.4529725
ACATGGG11250.038.433983
GTATCAA4650.038.407031
ATACACT500.001696815537.5984576
CATGGGA7450.035.327414
TGGATAG550.00270872734.180425
GGTACCT702.1749115E-433.5700578
CATGGGT1554.0017767E-1133.3534744
TCTTGGA1001.3988792E-632.898652
CATGGGG3050.032.359334
ATGGGAT1900.032.1565745
TTTAGGT600.0041483631.332052
Produced by FastQC (version 0.11.2)