FastQCFastQC Report
Sun 14 Apr 2019
SRR6576626_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576626_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences129891
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2700.2078665958380488No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG1510.11625131841313101No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG1320.10162366907637943No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGCT250.005226548856.3974694
GGTACCT558.7528497E-751.2704168
TAACGCC405.6592864E-446.9978834
GGGTACC652.766652E-643.3826647
CATGGGG3300.041.3011674
GTATCAA5350.040.471731
GTACATG12850.039.56111
TACATGG12900.038.9827422
ACATGGG13000.038.6828733
GAATAGA500.001684041237.6563031
ATGGGAG3050.036.9819455
ATAGTAC651.4069708E-436.1522183
ATGGGGA1850.035.5659645
GTATAAG550.002688371834.2330061
ACAGGGG550.002708781734.180283
TAGTACA550.002708781734.180284
CATGGGA8600.032.2427334
TTACACT600.004148442331.3319234
AACGCAG7350.030.0530686
TATAAAG953.7281294E-529.6828752
Produced by FastQC (version 0.11.2)