FastQCFastQC Report
Sun 14 Apr 2019
SRR6576628_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576628_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283537
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7080.2497028606495801No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3900.1375481859510399No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3880.13684281063847048No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3550.12520411798107478No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3220.1135654253236791No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3160.1114492993859708No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3130.11039123641711664No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3050.10756973516683889No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2890.10192673266628341No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG2850.10051598204114454No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGTA451.6029304E-552.2656671
TGGGTTA500.00170028937.598096
ACGTAGC500.00170028937.598093
TACGTAG651.4039192E-436.183922
TACATGG17150.033.1879352
GTACATG17800.031.9760171
ACATGGG17200.031.969313
CATGGGT3300.029.9075764
TACTGGT804.7733224E-429.3735127
ATGGGGA3850.029.2972165
CCGTCGT650.00614797928.9216129
CATGGGG6850.028.816064
GTATCAA10000.028.2234611
GGTACCT2700.027.850448
GGGTACC2700.027.850447
GTGTAGG1555.014408E-827.3130261
ATGGGTA2305.456968E-1226.5638715
AACGCAG10000.026.3186666
CAACGCA10600.025.2723065
CATGGGA8750.025.2444344