FastQCFastQC Report
Sun 14 Apr 2019
SRR6576628_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576628_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283537
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6180.21796097158395555No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3900.1375481859510399No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3400.11991380313680401No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3360.11850305251166514No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3160.1114492993859708No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3070.10827511047940834No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3070.10827511047940834No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2920.10298479563513757No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGGG18850.031.4188633
GGTTAGC600.004157767631.3302178
GTACATG19550.030.6261581
TACATGG19850.030.072842
CATGGGT3950.029.7491254
CCTAGGT650.006144145528.9253033
CTATTAA856.681879E-427.7323471
ATGGGTA3100.027.2924215
CTATGCA700.00882168826.859214
TGCACGG700.00882168826.859215
CGCTGAT350.00883659826.85447334-35
GGGTTAG700.00882928226.8544737
CTAGTAC909.4956736E-426.113123
CATGGGC3850.025.6383364
CTCGATA551.0175229E-425.63607464-65
GGTACCT2800.025.1760678
CATGGGG7300.025.111524
CTTTATA1501.1565935E-625.0685962
TAGTACT950.001301579424.7387454
GTATCAA10700.024.6740151
Produced by FastQC (version 0.11.2)