FastQCFastQC Report
Sun 14 Apr 2019
SRR6576630_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576630_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences323134
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7540.23333972902882397No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5370.16618492637729237No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4090.12657287688698807No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3760.11636039537776896No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3650.11295623487469594No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3560.11017101264490892No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3470.1073857904151219No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3320.10274375336547685No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3310.10243428422883386No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3250.10057746940897584No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGATA250.005233666456.39721394
GTATCAA9000.035.6361961
GTACATG17350.034.2527921
TACATGG17800.033.268022
AACGCAG9850.032.44516
ACATGGG18250.031.9326693
ACACCGT600.00415760231.3317876
CATGGGG8050.030.358754
GTCCTAG1401.8188075E-830.3207151
ATCAACG10650.030.0079083
TCAACGC10700.029.8676854
TCCTAGG804.7747474E-429.3735492
CAACGCA10800.029.1559685
GGTACCT3250.028.921658
ACGCAGA11200.028.5343047
CGCAGAG11950.026.743458
CATGGGT3950.026.175924
TATACTC1101.0164594E-425.6350985
GCGCTCA950.001302932224.7356229
TATCAAC13200.024.5669692
Produced by FastQC (version 0.11.2)