FastQCFastQC Report
Sun 14 Apr 2019
SRR6576631_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576631_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences296769
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7950.2678851227722572No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4650.15668752464037686No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4130.13916547887414116No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4100.1381545916183968No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4100.1381545916183968No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3780.12737179422379022No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3650.1229912827822313No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3510.11827380892209091No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3420.11524114715485782No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3320.11187152296904326No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2990.10075176315585523No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAGTG550.002706696734.2000279
TTGCCCG550.002715661434.176984
CAGTGCG600.00414523131.3500279
TAGTACT753.27245E-431.3288964
GTACATG20250.030.6533571
ACATGGG20550.029.4994723
TACATGG21150.029.3291782
CCTAACC650.00613093628.9384848
ATAGTAC650.006151168628.918983
GTATCAA12200.028.5233841
CATGGGG7750.027.2864574
GTGTATG1406.312148E-726.8714521
AACGCAG12850.026.6966086
GACCCTT909.5088506E-426.1074126
ATAACGC909.5088506E-426.1074123
GAATAAC950.00129826924.7500211
GTAAGGC1151.3751701E-424.5182673
CATGGGT4800.024.47574
TCAACGC14250.024.4035634
CGCGTGC1351.4467256E-524.3833549
Produced by FastQC (version 0.11.2)