FastQCFastQC Report
Sun 14 Apr 2019
SRR6576632_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576632_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences448545
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7880.17567914033151635No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5540.12351046160362952No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4810.10723561738510072No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12000.038.8843541
GTACATG28750.036.886341
TACATGG29350.036.0315632
ACATGGG29250.035.5119973
CCGTCTA550.002716403234.178869
CATGGGA16350.029.321834
AACGCAG15550.029.3190576
TCAACGC15250.029.2794174
ATCAACG15450.028.9003963
CAACGCA16000.028.2007035
CATGGGT5950.027.6477494
ATGGGAG6800.027.6477475
CATGGGG10500.027.3054434
ATGGGAC3700.026.6763425
GTCCTAT1257.836346E-626.3942261
TTAGACC909.507218E-426.1117614
GTATTCG459.520854E-426.10884956-57
TATCAAC18000.025.8506432
ACGCAGA18500.024.641117
GTTATAT1551.5058704E-624.3264751
Produced by FastQC (version 0.11.2)