FastQCFastQC Report
Sun 14 Apr 2019
SRR6576634_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576634_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences409530
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9660.23588015529997802No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5050.12331208946841501No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4590.11207970112079702No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4480.10939369521158401No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4370.106707689302371No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4220.10304495397162601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10200.040.0882341
ATAGTAC853.9473525E-738.7058833
GTACATG22250.036.543821
TACATGG22100.036.1538432
ACATGGG22200.035.990993
ATCAACG11750.034.43
AACGCAG12300.034.008136
CAACGCA12050.033.933615
TCAACGC12100.033.4049574
ACGCAGA14000.029.8785727
CGCAGAG14600.028.6506868
CATGGGA10750.028.4186044
CATGGGG8450.028.3668654
TATCAAC14950.027.6655522
CCTTATA856.8085437E-427.647062
GTCGTGC700.00882950126.8571438
ATGGGGG3700.026.6756745
CATGGGT5850.025.7094044
CATGGGC4050.025.5308634
ATGGGAT3550.025.154935
Produced by FastQC (version 0.11.2)