FastQCFastQC Report
Sun 14 Apr 2019
SRR6576635_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576635_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363111
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7110.19580789345406777No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4910.13522035961455314No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4680.12888620834951298No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4600.12668302530080333No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4370.12034887403576316No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3930.10823136726786024No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3820.1052019905758845No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10850.040.7798961
TACATGG18900.037.7930152
GTACATG19250.037.4118081
ACATGGG19450.036.482713
CATGGGT4950.035.125814
GGGTACC3700.031.751787
AACGCAG13650.031.6726916
GGTACCT3600.031.3500068
ATCAACG13900.030.7649633
TCAACGC14500.030.1401024
ATGGGTA3600.030.0230715
CATGGGG8350.029.827664
CAACGCA14350.029.8002055
ACGCAGA14850.029.113287
ATAAGAC1005.3193286E-528.195583
CGCAGAG15500.027.9116178
TATCAAC15900.027.4862562
CAGCGTA350.00884067326.85293436-37
CATGGGA9950.026.4481144
TACTACT1101.01754165E-425.6323434
Produced by FastQC (version 0.11.2)