FastQCFastQC Report
Sun 14 Apr 2019
SRR6576637_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576637_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences304939
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5710.18725056486707178No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3870.1269106280272448No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3820.12527095583051037No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3190.10461108615165655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8150.042.2485961
TACATGG17550.038.8364452
GTACATG18350.038.042821
ACATGGG19100.035.6848033
ATCAACG10000.034.314013
CAACGCA10200.033.180355
TCAACGC10250.033.0184944
ATGGGAG4450.032.74545
AACGCAG10250.032.5599026
ACGTTAA300.004160423331.33185692-93
TATCAAC11600.031.2019232
CATGGGA10850.029.8928954
ATATAAC953.7403508E-529.6876833
CATGGGT4600.029.63394
TAGTACT804.7694758E-429.3784354
GGTACCT2250.029.2430698
ACTAGAG650.006143663528.9264582
CATGGGG6900.027.9308014
GTATATA1205.6653407E-627.5146431
ACGCAGA12350.027.018977
Produced by FastQC (version 0.11.2)