FastQCFastQC Report
Sun 14 Apr 2019
SRR6576638_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576638_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269879
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5160.19119679560099156No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3910.14487974240307694No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3680.13635740461466064No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3230.11968326546341138No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2990.11079039124941177No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2740.10152698060982884No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACGG205.661351E-447.02132468-69
ATGGGTA2250.045.9423265
CATGGGT3350.040.6748624
GTACATG15500.038.880981
GTATCAA8600.038.8703841
GTGCGCG500.001685147437.6659471
TACATGG16150.036.3807222
GGTACCT2350.036.0163358
GGGTACC2350.035.989647
ACATGGG16200.035.964963
TGGGTAC2450.034.520676
GTCTTAT1052.0296702E-631.388291
TATAGTA600.004153706631.335932
GTTATTC600.00416125131.3243163
TCAACGC10700.030.7388134
AACGCAG10400.030.7219246
ATCAACG10700.030.2996863
CAACGCA10750.030.1587625
GTACCTG3050.027.750299
ACGGTTC350.00883636126.8543944-45
Produced by FastQC (version 0.11.2)