FastQCFastQC Report
Sun 14 Apr 2019
SRR6576641_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576641_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences442927
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8500.19190521237133884No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5550.12530281513658006No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4850.10949885647070509No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4730.10678960641369796No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4700.1061122938994462No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4540.10249996049010333No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12100.041.182651
GTACATG25750.039.4339561
TACATGG25700.039.327762
ACATGGG26100.037.6358343
CATGGGT6350.037.0076984
ATGGGTA3850.036.62325
ATCAACG15150.032.57413
GGGTACC3900.032.5383077
AACGCAG15600.032.2370266
CAACGCA15550.032.0384335
TCAACGC15450.031.9415954
GCATTAC902.5864429E-531.3402611
CATGGGA13700.031.2188284
ATGGGGA5450.031.0457215
GTATAAT2000.030.5567531
GTAATAG1102.9579169E-629.91571
CATGGGG9550.029.5286524
GGTACCT4350.028.0918188
ACGCAGA17950.028.0165797
TATAATG2350.028.006192
Produced by FastQC (version 0.11.2)