FastQCFastQC Report
Sun 14 Apr 2019
SRR6576642_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576642_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404127
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8670.21453651946046665No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5050.12496071779415877No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5000.12372348296451363No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4870.12050667240743629No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4750.11753730881628795No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4140.10244304389461728No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG405.6693767E-447.004951
GTATCAA12300.041.272641
ACATGGG25600.035.7912643
TACATGG25650.035.7347562
GTACATG26000.035.6152881
TATACTG2000.035.2406275
AACGCAG14550.033.908516
GTTATAG702.1811549E-433.5749631
ATCAACG15050.033.40643
TCAACGC15300.032.860544
CAACGCA15300.032.5534325
TAGACCC753.2769187E-431.3250034
CCTATAC753.2769187E-431.3250033
CCCCGAT1401.882654E-830.2174661
TTATACT1252.3622852E-730.0724
CATGGGT5350.029.8612174
CATGGGG10100.029.774264
ACGCAGA16950.029.1073047
CATGGGA14500.028.5165564
TATCAAC18350.028.4335122
Produced by FastQC (version 0.11.2)