FastQCFastQC Report
Sun 14 Apr 2019
SRR6576642_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576642_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404127
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7770.19226629252685418No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5430.1343637024994618No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5310.13139433890831348No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4820.11926943757779114No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4410.10912411197470102No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13450.040.23541
TACATGG25900.034.4742742
ACATGGG25600.034.3275573
AACGCAG15350.034.2886926
GTACATG26650.034.079471
ATCAACG15600.033.7391933
CATGGGT5450.033.6286434
CAACGCA15750.033.4178665
TCAACGC16050.032.7932324
TATCAAC18450.029.2915762
ATGGGTA3400.029.0256295
CAGTACG650.006138559428.933639
ATGTTAC1005.2706713E-528.2347551
ACGCAGA19050.027.6289427
CGCAGAG19400.027.1439198
CATGGGG8900.026.9290754
CATGGGA15200.026.897814
ATGGGAG5700.024.733625
AGGACGG1351.45587255E-524.3671955
GCAGAGT22600.023.9246549
Produced by FastQC (version 0.11.2)