FastQCFastQC Report
Sun 14 Apr 2019
SRR6576643_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576643_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences579176
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG15020.25933395030180806No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA10250.1769755652858544No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA9430.16281752006298603No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA8430.14555161125461No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT7190.12414188433222371No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG7010.12103402074671603No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6960.12017072530629723No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG6870.11861679351354339No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA6720.11602690719228698No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT6600.11395499813528186No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG6400.11050181637360665No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6080.1049767255549263No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC6030.1041134301145075No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT5960.10290481649792117No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG5870.10135088470516734No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA12350.050.247551
GTACATG34100.037.3613781
ACATGGG33950.036.9629753
ATAGGGG906.1834726E-736.5553553
TACATGG35250.036.2758642
CATGGGT7800.036.1536454
ATGGGTA5400.035.684995
ATCAACG17650.034.08483
AACGCAG18150.033.9226766
TCAACGC17900.033.6087534
CAACGCA18250.032.9642035
GGTACCT5450.032.7704628
GTATAGG1052.0589487E-631.3412781
GGGTACC6000.029.7665047
ACGCAGA21100.028.9571867
CATGGGA13850.028.8446084
CATGGGG17850.027.3836024
CGCAGAG22550.026.886778
CATATAG700.00883270526.8569973
ATGGGAG7350.026.8569935
Produced by FastQC (version 0.11.2)