FastQCFastQC Report
Sun 14 Apr 2019
SRR6576643_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576643_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences579176
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG13340.23032722350373636No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA10240.17680290619777064No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA8880.1533212702183792No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA7900.1364006795861707No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG6960.12017072530629723No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG6820.11775349807312459No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA6770.11689020263270578No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG6680.11533627083995193No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG6580.11360967995911433No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC6550.11309170269486306No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6490.11205574816636049No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6230.1075666118761827No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT5880.1015235437932511No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCATA250.005235664556.3974657
GTATCAA15250.052.2136271
ATCAACG20150.039.650823
AACGCAG19800.039.639636
GTACATG32500.038.707481
CAACGCA20400.038.7041445
TCAACGC20450.038.609514
TACATGG33550.037.822442
ACATGGG33100.037.6267053
ACGCAGA22500.034.6739967
CGCAGAG23700.032.7200478
TATCAAC24400.032.5518152
TAACGGT300.00416191131.33192454-55
GGTACCT5750.031.0594718
GCAGAGT25200.030.3994279
ATGGGTA6100.030.0478295
CATGGGG16600.029.7275774
ATATATG650.006078128228.9943681
GTATAAC650.006078128228.9943681
CATGGGT9100.028.9217764
Produced by FastQC (version 0.11.2)