FastQCFastQC Report
Sun 14 Apr 2019
SRR6576645_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576645_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239499
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5480.22881097624624738No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3670.1532365479605343No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2870.11983348573480473No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2670.11148272017837235No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2590.10814241395579939No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2430.10146180151065348No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACC503.0023954E-546.9995844
GGACTAT450.001012390641.777416
GTACATG16600.036.822281
TACATGG16600.036.822282
ACATGGG16950.036.046883
TATTAGT550.002707300234.1957972
GTATCAA7800.033.757391
CATGGGG7250.032.4135064
TATAGAC753.268034E-431.3330544
CATGGGT3500.029.5425954
GGACCGT650.006144841728.9228216
ATGGGAG2800.028.5354615
ATGGGGG2400.027.4164245
GTATAGA700.00880473626.8681241
GTCCAAA700.00880473626.8681241
CATGGGC2450.026.8569034
AACGCAG9600.026.4372656
GGTACCT2357.2759576E-1225.9997718
GTACTTG1458.571951E-725.9416371
CATGGGA8350.025.8919854
Produced by FastQC (version 0.11.2)