FastQCFastQC Report
Sun 14 Apr 2019
SRR6576646_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576646_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224436
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5600.24951433816321802No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3870.1724322301235096No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3450.15371865476126825No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2970.13233171149013528No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2850.126984975672352No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2750.12252936249086599No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2700.12030155590012298No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT2680.11941043326382578No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2600.11584594271863694No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC2390.10648915503751627No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA2330.10381578712862463No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8000.044.6374051
TACATGG15950.044.4827462
GTACATG16000.044.050071
ACATGGG16850.041.2702523
CATGGGT3600.039.1556174
CATGGGG6850.039.0984574
AACGCAG9000.038.1114656
ATCAACG9700.035.8455543
CAACGCA9600.035.7295045
TCAACGC9900.035.1214074
CTAGTCT550.002716126934.1721764
ACGCAGA10400.033.4328737
CGCAGAG10450.033.272918
ATGGGGG2450.032.6030435
CCGTAAT300.004164049431.32449564-65
ATGGGGA3350.030.8569645
GCAGAGT11300.030.3542679
TATCAAC11700.029.3165132
CCTAATA650.006152236428.9149172
TTTGGAC650.006152236428.9149173