FastQCFastQC Report
Sun 14 Apr 2019
SRR6576648_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576648_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences399417
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8960.22432695653915583No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5040.12618391305327514No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4390.10991019410791228No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4130.10340070652976713No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4030.10089705746124976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13450.036.3420071
CATGGGT5650.034.1061944
TACATGG28450.033.701232
GTACATG28450.033.3708271
ACATGGG28050.033.1764683
CATGGGG8800.030.4431824
TGCACCG804.7753635E-429.3755
ATGGGGA5200.028.9230775
GTATACC650.00614942528.9230773
AACGCAG16650.028.7927936
TCAACGC17250.028.0637684
ATCAACG17350.027.9020163
CAACGCA17200.027.8720935
CCTATAC1205.8306778E-627.4166663
ATGGGTA4000.027.0255
CAACTCG700.00882924526.8571434
GATATAG909.5067703E-426.111111
ACGCAGA19250.024.9038967
CGCAGAG19350.024.7751928
TATCAAC20350.024.4815732
Produced by FastQC (version 0.11.2)