FastQCFastQC Report
Sun 14 Apr 2019
SRR6576650_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576650_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences365555
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8890.24319185895419296No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4500.1231004910341809No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA4140.11325245175144642No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4100.11215822516447593No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA3830.10477219570242507No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3730.10203662923499884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGT5400.036.5550234
ACATGGG22900.035.711273
GTATCAA13500.034.1180231
GTACATG24400.034.0937651
GGTACCT3750.033.8395088
ATGGGTA4050.033.653835
TACATGG24450.033.639592
AACGCAG14400.032.312036
CATGGGG10050.031.3328764
CCGTGTA600.004157601431.3328769
GTCTTAC600.004157601431.3328761
CAACGCA14900.031.2277365
GGGTACC4100.030.9507717
ATCAACG15400.029.9086553
TCAACGC15400.029.9086554
TGGGTAC4300.029.5111986
TATAGGA1452.6500857E-829.171992
ATAAGGG650.006149192328.9226533
ACCGTGT650.006149192328.9226538
GTATAGG1154.186584E-628.6082781
Produced by FastQC (version 0.11.2)