FastQCFastQC Report
Sun 14 Apr 2019
SRR6576651_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576651_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328843
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8980.273078642391658No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5130.15600149615469996No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4530.13775570713075846No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4330.13167377745611128No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3800.11555666381829627No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3800.11555666381829627No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA3590.10917063765991675No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3590.10917063765991675No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3580.10886654117618437No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA3570.10856244469245202No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3520.10704196227379022No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT3410.10369690095273429No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3400.10339280446900193No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3330.10126412908287542No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3320.10096003259914305No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTAC651.4036147E-436.1873931
ACATGGG20150.034.5163463
GTACATG21100.033.6662831
TACATGG20950.033.6469232
TGTAATA702.1833867E-433.5668142
GTTGTAT753.2527503E-431.362411
CATGGGC3400.030.4075854
CATGGGG8150.029.9836064
GTATCAA11200.029.822291
CCTTACA1154.188905E-628.6047612
CATGGGT4900.027.81254
AACGCAG12050.027.2991496
GTGTTAC700.00878716626.8820651
GTATACT700.00878716626.8820651
ATGGGTA3350.026.6530515
GTATAAG1101.0097834E-425.6601521
ATCAACG12850.025.5995923
TCAACGC12950.025.4019134
CAACGCA13150.025.0155725
ATGGGGA4400.024.5648025
Produced by FastQC (version 0.11.2)