FastQCFastQC Report
Sun 14 Apr 2019
SRR6576652_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576652_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences353305
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6660.1885056820594104No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4530.12821782878815755No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA4020.1137827089908153No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC250.00523111456.4052663
GTATCAA10050.040.79431
AATAGAC651.410734E-436.157223
CGCGGTA959.419182E-734.6348194
ATCAACG12500.031.9629863
ATGGGAG3850.031.7432255
GTACATG20250.031.6490121
ATAACGC1052.0585157E-631.3362623
TCAACGC13050.031.336264
CAACGCA12750.031.3362585
TACATGG20650.031.1845112
AACGCAG13300.030.747236
ACATGGG20850.030.6599353
ACTAGGC856.8029674E-427.649643
TATCAAC15000.027.2625452
GTATACT700.0088238726.8596524
CATGGGA10350.026.3406224
ACGCAGA15600.026.2102767
GTAATAG909.3589677E-426.1802481
GTACTAG909.3589677E-426.1802481
Produced by FastQC (version 0.11.2)