FastQCFastQC Report
Sun 14 Apr 2019
SRR6576654_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576654_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282559
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4750.16810648395556327No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3270.11572804263888249No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3110.11006550844248458No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGA200.002161351770.4994664
TGCCCGC601.5969927E-647.016291
GTATCAA7050.038.0131651
ACCGCTC500.001699919137.5997168
ACCGTCG500.001699919137.5997168
GTAAACC757.4240943E-637.5997163
GTACATG16150.036.681441
TACATGG16250.036.4557042
ACATGGG17350.033.8614163
CATGGGA8850.032.9263084
ATCAACG8550.030.7833923
CAACGCA9050.029.6019885
AACGCAG9250.028.9619456
CACCGTC650.0061466528.922867
TCAACGC9050.028.563324
ATGGGAG3950.028.5567475
GTGTTAC856.789514E-427.6566371
ATGGGTA2253.6379788E-1227.1553525
TATAGTG700.00882527626.8569417
CTAATAG700.00882527626.8569413
Produced by FastQC (version 0.11.2)