FastQCFastQC Report
Sun 14 Apr 2019
SRR6576654_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576654_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences282559
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4450.15748923233731715No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3520.12457575232075425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA7050.045.4594841
TACATGG15250.037.5978782
GTACATG15200.037.518591
ACATGGG15700.035.3228453
CATGGGG5200.031.632834
ATCAACG10150.030.5598533
CATGGGA8800.030.4414624
TCAACGC10250.030.2617054
AACGCAG10100.030.2458156
CAACGCA10300.030.1148035
GTATTAT856.6936074E-427.7239951
TATCAAC11700.027.3146952
AGGCGAT350.008834721526.85562754-55
ACGCAGA11700.026.1096347
CGCAGAG11900.024.8809478
ACCATAC950.001302581324.7354473
ATGGGAG4200.024.6176595
GCGCAAA1201.8339758E-423.49867294
GTCTTAA1652.5887584E-622.851291
AATATAG1050.00233193822.3796885
Produced by FastQC (version 0.11.2)