FastQCFastQC Report
Sun 14 Apr 2019
SRR6576655_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576655_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256536
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4690.18282034490285964No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3030.11811207783702872No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA2890.11265475410858515No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2780.10836685689337949No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACT200.00216002770.5084841
TGCCCGC408.010498E-658.757071
GTACATG15500.041.243671
TACATGG16350.038.524512
GTATCAA7700.038.459171
ACATGGG16650.037.2512173
GTATAGG550.002711491234.1859321
GTTATAC550.00271668234.1726043
CATGGGG5400.033.9352954
CATGGGT3550.033.089674
ATGGGTA1850.033.018125
AACGCAG8800.032.0368166
ATCAACG8950.032.0248833
TCAACGC9100.031.4974
GTAATAA753.266199E-431.3371031
GTATACG600.00415256531.3371011
TGGACCG902.5909063E-531.3248865
ATGGGAG3250.030.3610465
CAACGCA9550.030.012855
CATGGGA9300.029.8091664
Produced by FastQC (version 0.11.2)