FastQCFastQC Report
Sun 14 Apr 2019
SRR6576655_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576655_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256536
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG4630.18048149187638382No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3770.14695793183023045No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2940.11460379829731501No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2690.10485857735366576No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2650.1032993420026819No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTATG250.00521914256.4334758
GTATCAA8300.043.662361
GTACATG16400.041.8989941
TACATGG16450.041.7146342
ACATGGG17200.039.88793
ATGGGAG3550.037.0635035
AACGCAG9900.035.5994156
CATGGGG6400.035.243424
GGTTATA550.0026948734.2288171
GGTACCT1703.6379788E-1233.1961638
ATCAACG10800.032.6327973
CATGGGT3750.032.5805854
CAACGCA10850.032.4824145
TCAACGC10850.032.4824144
ATGGGTA2200.032.0394745
CACGTAT600.004158810231.3274867
ATGGGGG2600.030.7250335
CATGGGA9050.030.6351664
CGCAGAG11700.030.5480378
TATCAAC11700.030.5301692
Produced by FastQC (version 0.11.2)