FastQCFastQC Report
Sun 14 Apr 2019
SRR6576656_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576656_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences420124
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12230.2911045310432158No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7140.16994982433757652No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA6500.15471622663784979No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA6090.14495720311146235No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5880.1399586788662395No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5500.13091373023202674No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG5410.12877150555550265No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4990.11877445706505699No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4970.11829840713694052No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4600.109491483466786No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4460.10615913396997077No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4290.10211270958098086No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4280.10187468461692263No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4260.10139863468880617No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10600.044.9298551
TATAGTA550.002714330334.1836782
ATACCGT1256.2445906E-933.8418436
TCAACGC14350.033.737034
GTATAGG702.1410792E-433.6815071
ATCAACG14250.033.6439363
CAACGCA14850.032.2845845
AACGCAG15150.032.265786
GTACATG25000.031.6875611
ACATGGG25350.031.5204563
CATATAA1052.009243E-631.4360711
TACATGG25850.031.0926062
ACGCAGA16700.029.2710557
ATGGGAG3900.028.924655
TATCAAC17450.028.0129852
CGCAGAG17600.027.7742398
CATGGGG10700.027.6744064
TACCGTC1555.0646122E-827.2918117
GTATTAC700.0086898926.9452041
GTTAGAC1057.407814E-526.8586043