FastQCFastQC Report
Sun 14 Apr 2019
SRR6576657_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576657_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences374361
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG12070.32241606363910774No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6870.18351270565042832No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5970.15947173984469537No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5750.15359505931440506No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5350.1429101856229682No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4960.13249243377381725No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4950.13222531193153134No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG4930.13169106824695948No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4820.12875272798181434No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4620.1234102911360959No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4560.12180756008238038No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC4400.11753361060580564No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4370.11673224507894786No Hit
GCATATGCTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGC4150.11085556454865758No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3960.10578024954522507No Hit
CGGCCGGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCC3880.10364327480693769No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA9200.042.4407121
ACATGGG20700.041.771073
TACATGG22150.040.0973972
GTACATG22300.039.6593321
GTACTAG500.001692987737.634181
TACTAGT500.001701903437.5939644
ATGGGTA3600.036.5496865
CATGGGT5150.035.5865174
AACGCAG11300.034.5165826
CATGGGG9750.033.738174
ATCAACG11200.033.566043
CAACGCA11600.033.2188075
TCAACGC11500.033.0990334
GAACGTT300.00416333431.32830246-47
CTATAGG1252.3372922E-730.1073421
GGTACCT4350.029.1872228
GGGTACC4250.028.7483257
ACGCAGA13500.028.5435667
CATGGGA9550.028.0478534
CGCAGAG13650.027.9042688
Produced by FastQC (version 0.11.2)