FastQCFastQC Report
Sun 14 Apr 2019
SRR6576659_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576659_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496204
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9950.20052236580116242No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6190.12474707983006988No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5380.1084231485437441No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT500.001701345937.5988736
TACATGG29500.035.0674632
GTACATG29800.034.556641
ACATGGG29850.034.3273853
GTATCAA13650.032.381641
CATGGGG11500.031.8773044
GTATAGA902.5825972E-531.3481881
CATATAG804.7750346E-429.3770793
ATGGGTA5900.027.8805185
TACACCC856.811787E-427.6462294
AACGCAG15900.027.4897386
ATCAACG16600.026.3331873
CAACGCA16550.026.1261045
TCAACGC16550.026.1261044
CATGGGT9000.026.1103274
AACGCCG1751.6427293E-724.1707025
CATGGGA14600.024.143114
TGGGTAC6300.023.87236
GGTACCT5750.023.7036368
ATGGGAG6600.023.4992945
Produced by FastQC (version 0.11.2)