FastQCFastQC Report
Sun 14 Apr 2019
SRR6576660_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576660_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457352
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9560.20902936906365335No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6440.14081057916003428No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5200.1136979831726985No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5110.11173013346394026No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4930.10779443404642378No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4720.10320278472598786No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4610.10079763508195No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG550.002712967634.1878241
ACATGGG28050.034.005363
TACATGG28700.033.249742
ATGGGAG5900.032.652475
GTACATG29600.032.5563931
GTATCAA14300.031.2292631
CATGGGG11400.030.9129624
CATGGGA13350.029.2133264
TCTATAC650.006158215528.915513
GTCTAGA1205.8251353E-627.4214841
AACCTTA700.0088418426.8501136
AACGCAG17700.025.7503226
TATATAC1301.0881682E-525.3010713
GCACCGT1151.3778835E-424.5153226
CAACGCA18500.024.128825
ATCAACG18700.023.8707563
TCAACGC18700.023.8707564
TAACCCT1401.9319308E-523.4938495
TTACTCC1252.42463E-422.5540964
TAGTAGT1252.42463E-422.5540964
Produced by FastQC (version 0.11.2)