FastQCFastQC Report
Sun 14 Apr 2019
SRR6576660_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576660_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences457352
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9850.2153702181252077No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6590.14409032867463137No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5910.12922213087512463No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5280.11544718291381693No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5130.11216743339921985No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5110.11173013346394026No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4910.10735713411114416No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4820.10538928440238592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG28250.037.9968761
TACATGG28500.037.593572
ACATGGG27750.037.424233
GTATCAA14150.037.264311
CATGGGG10400.033.8884354
CATGGGT5350.030.7424054
AACGCAG16900.030.5864876
ATAGTGC650.006155485828.9181336
CAACGCA17600.028.8359785
TCAACGC17600.028.8359784
ATCAACG17300.028.7927653
ACGCAGA18700.027.6423327
CGCAGAG19450.026.5967868
ATGGGCG1258.020754E-626.31555
CATGGGA15550.025.9891244
ATGGGAT3350.025.2494145
CATGGGC5400.025.2364254
ATGGGAG6200.025.011855
GTATATA1901.3047611E-824.7786661
TATCAAC21650.024.0928782
Produced by FastQC (version 0.11.2)