FastQCFastQC Report
Sun 14 Apr 2019
SRR6576661_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576661_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416847
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10600.25428994331253435No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA7040.16888690574719262No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5850.14033926116776657No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5750.13794029943840308No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG5370.12882424486682165No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5090.12210715202460375No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4960.11898850177643117No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4500.10795327782135891No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4500.10795327782135891No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4490.10771338164842256No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG4290.10291545818969551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8950.055.150931
TCAACGC12400.038.659914
ATCAACG12350.038.4358753
CAACGCA12650.037.524355
AACGCAG13050.037.094466
GTACATG24050.036.9430961
TACATGG24900.035.3043982
ACATGGG24500.035.1048663
CATATAG851.7525395E-533.1752853
GGTATCA3750.032.5933231
CATGGGT7850.031.731354
ATGGGTA4600.031.6727835
ACGCAGA15450.031.0280177
TATCAAC16350.029.614612
CGCAGAG16300.029.4099928
GGTACCT4650.029.3107818
ATGGGAT4050.027.8508575
GGGTACC4900.027.815337
CATGGGA12750.027.646074
GCAGAGT18050.026.5586079