FastQCFastQC Report
Sun 14 Apr 2019
SRR6576662_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576662_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377586
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG11200.2966211670983564No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6880.18221014550327608No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5890.1559909530544035No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5760.15254802879344043No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4840.12818271863893257No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4830.12791787831116622No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4580.12129687011700645No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT4380.11600006356167866No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4270.11308681995624839No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4260.11282197962848199No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3900.10328772782889196No Hit
GATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAGTCCTGTAT3790.10037448422346167No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10450.045.0038571
GTACATG23850.042.0007671
ACATGGG24650.040.7963
TACATGG24850.040.4998472
TCAACGC12300.037.4405064
AACGCAG12850.036.9301836
CATGGGA13050.036.729114
ATCAACG12800.036.3451083
CAACGCA12850.036.1988955
TCCGACC550.002718837734.1712278
ATGGGTA4200.032.442325
AGATCGT300.004166402431.32362210-11
GGCGTTA300.004166402431.32362242-43
TCCGTTG300.004166402431.32362236-37
CATGGGT6800.031.0974184
ATGGGAT5050.030.7033525
CGCAGAG15450.030.4112828
ACGCAGA15500.030.313187
CATGGGG7250.029.8153954
CCGACCA650.006158326328.914119