FastQCFastQC Report
Sun 14 Apr 2019
SRR6576663_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576663_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480946
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9510.19773529668611445No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6000.12475413040133404No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5200.10812024634782283No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4900.10188253982775612No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4890.10167461627708724No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4870.10125876917574946No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4810.10001122787173614No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA10150.047.6990661
ATCAACG12700.038.117733
GTACATG26050.037.5313571
TCAACGC13000.037.2380944
CAACGCA13100.036.9538275
TACATGG26700.036.4416282
ACATGGG26950.035.4022453
AACGCAG13950.034.7021646
GGTATCA5350.031.629141
TATCAAC15800.030.6421222
ACGCAGA15950.030.0561267
CATGGGA14150.029.5615434
CGCAGAG16400.029.2314178
ATGGGAG8350.027.5805635
CATGGGT7100.027.1405744
CATGGGG9700.027.1337534
ATCGCGT350.00883574726.85687488-89
ATGGGTA4950.024.6866255
ATGGGAT4300.024.0462745
GCAGAGT20450.023.9019629
Produced by FastQC (version 0.11.2)