FastQCFastQC Report
Sun 14 Apr 2019
SRR6576663_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576663_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480946
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9170.1906658959633722No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5950.12371451264798958No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5770.11997188873594955No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5110.10624893439180282No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5090.10583308729046503No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5070.10541724018912725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11050.047.7101481
TCAACGC13800.037.4619874
ATCAACG14000.036.9268153
GTACATG26950.036.85351
TACATGG26700.036.7885132
ACATGGG27000.036.379753
CAACGCA14050.035.791895
AACGCAG14050.035.791896
CATGGGG11300.031.6091184
TATCAAC16750.031.425372
CATGGGA13700.029.8452974
ACGCAGA16850.029.5653577
CGCAGAG17400.028.9009258
CATGGGT7050.028.6653044
GGTATCA5200.028.0616651
ATGGGTA4200.027.974865
ATTACTC856.8121776E-427.6457423
TGGGTAC4600.026.5639536
GGTACCT4450.026.403248
TCACACG909.5122424E-426.1098699
Produced by FastQC (version 0.11.2)