FastQCFastQC Report
Sun 14 Apr 2019
SRR6576664_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576664_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433719
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9840.22687500432307553No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5530.12750190791733818No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5100.11758765467964281No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5000.11528201439180669No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4750.10951791367221635No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4510.10398437698140962No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGAACG200.002164584670.479331
GTATCAA10850.048.934961
GTACATG25800.041.1584741
ATCAACG13000.040.1190073
ACATGGG25900.039.911083
TACATGG26100.039.7852672
CAACGCA13500.038.6331185
TCAACGC13500.038.6331184
AACGCAG13650.038.55286
CATGGGT7600.035.8579064
ACGCAGA15550.033.8421677
CGCAGAG15650.033.6259238
TATCAAC16450.033.1331442
GGTACCT3600.032.6293228
ATGGGTA3950.032.1171655
CATGGGA11950.031.8484024
GGGTACC3700.031.7474487
ATCGTGA300.00416639831.32414854-55
CATGGGG11450.029.5459234
TGGGTAC4050.029.0038436
Produced by FastQC (version 0.11.2)