FastQCFastQC Report
Sun 14 Apr 2019
SRR6576664_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576664_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433719
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG9630.22203315971861967No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5610.12934642014760708No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5240.12081555108261341No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5090.1173570906508592No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4690.10813452949951466No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4620.10652058129802935No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA4490.10352324892384239No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4380.10098704460722265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA11300.054.1222531
AACGCAG14200.043.350386
ATCAACG13900.043.2718163
CAACGCA14200.043.019465
TCAACGC14150.042.8393864
GTAATAC1003.324749E-837.5923923
TACATGG25450.037.4816062
GTACATG25400.036.8578831
ACGCAGA16800.036.6413967
ACATGGG25850.035.9927143
TATCAAC17200.035.5160682
CGCAGAG17500.035.200098
GTTAGCA1651.8189894E-1234.2025079
TAATGCG501.4128673E-632.8933418-19
TAATACT1151.13655915E-732.6890344
GGTTAGC1351.3169483E-831.348688
GGTATCA5400.030.4919571
CATGGGA13200.030.2590264
CATGGGG9200.029.6244374
GCAGAGT21000.029.3367929