FastQCFastQC Report
Sun 14 Apr 2019
SRR6576669_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576669_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187257
Sequences flagged as poor quality0
Sequence length100
%GC47

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG5140.2744890711695691No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3220.17195618855369893No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA2520.1345744084333296No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA2500.13350635757274762No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA2220.11855364552459988No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA2110.11267936579139898No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT1880.1003967808947062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACAG601.6000522E-646.990795
CCGCTAA405.66996E-446.9907884
ATGGGTA2100.042.5154765
CCCGCTA450.001012756541.769593
CATGGGT2850.041.2199864
GGGTACC1600.041.116947
CTCGATT608.8059685E-539.15899394
GCGTTAA250.001699968637.60267328-29
GTATCAA8000.037.0250171
ATAGCCC651.4106026E-436.146763
CGCTAAA550.002713837234.175125
GATTGGC702.1805606E-433.564859
GGTACCT2000.032.8935558
AATCGTA300.004155986531.33556244-45
TGGGTAC2100.031.3271926
TACATGG15350.030.3148572
CAACGCA9950.030.2252335
TCAACGC9950.030.2252334
GTACATG15800.029.7568971
AACCGCG404.772814E-429.37708740-41
Produced by FastQC (version 0.11.2)