FastQCFastQC Report
Sun 14 Apr 2019
SRR6576672_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576672_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337389
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG10490.3109170719851566No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6150.18228217280349981No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT5350.15857067065019903No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA5250.15560673288103644No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5090.15086443245037628No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4090.12122505475875028No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG4060.1203358734280015No Hit
CTCTAGATAACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCC3860.1144079978896763No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG3790.11233324145126249No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT3750.11114766634359745No Hit
GATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACAGTGAAACTGCG3570.10581257835910478No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3380.10018109659769582No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8000.044.06251
TACATGG19100.033.2198942
GTACATG19300.033.1191671
GTATATG1151.1332668E-732.6956521
AACGCAG10750.032.353496
ATCAACG10700.032.065423
ACATGGG19800.032.0454563
TCAACGC10850.031.6221184
CAACGCA11000.031.6181835
GGGTATC650.00614809128.9230777
CGCAGAG12050.028.863078
CATGGGT5450.028.4587174
ACGCAGA12300.027.8943127
ATGGGAG3900.027.7179495
GTAATAC1205.8271544E-627.4166663
ATGGGTA4300.027.3255835
GTATTAT1057.4063064E-526.8571431
CTATTAC700.00882733426.8571433
CATGGGG9000.026.6333314
TATTATG909.5036963E-426.1111132
Produced by FastQC (version 0.11.2)