FastQCFastQC Report
Sun 14 Apr 2019
SRR6576672_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576672_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences337389
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG8380.24837798505582576No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA5680.16835166528843562No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA5190.15382837021953888No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA4740.14049065025830718No Hit
GCTTTGAACACTCTAATTTTTTCAAAGTAAACGCTTCGGGCCCCGCGGGA4480.1327844120584844No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT4430.1313024431739031No Hit
GTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTG4340.12863489918165677No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG3750.11114766634359745No Hit
AGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCG3630.10759094102060233No Hit
TTCTAGAGCTAATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGG3560.10551618458218852No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3500.10373782192069096No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA8850.045.220821
AACGCAG10700.036.0180136
ATCAACG11050.035.3024983
CATGGGT5150.034.6789554
CAACGCA11350.034.3693925
TCAACGC11350.034.3693924
CATAAGA550.00271455334.181172
GTACATG17800.033.06381
GTATAAA1308.991265E-932.595821
ACATGGG18400.031.9287453
TACATGG18550.031.9239232
GTAAGAC600.00415723931.332743
ACGCAGA12650.030.4658247
TGGGTAC3750.030.0794326
GTCCTAA953.699755E-529.7365361
CGCAGAG13150.029.307438
TATCAAC13500.029.243892
GGTACCT3700.029.2156648
GGGTACC3900.028.9225277
ATGGGTA4100.028.6579935