FastQCFastQC Report
Sun 14 Apr 2019
SRR6576674_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR6576674_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319265
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG6590.2064116016475342No Hit
GTCCTGTATTGTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTA3690.11557796814558438No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA3580.11213255446102767No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT3400.10649460479538941No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3370.10555494651778304No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAC450.001010845641.794631
GTATCAA8250.039.324951
TACTAGG906.1521496E-736.57032
GTACATG20250.036.2220151
TACATGG20050.036.1143152
ACATGGG20550.034.3042833
ATACCCC550.002714971334.179544
CATGGGT5800.033.2219244
ATCAACG10450.031.0314243
AACGCAG10450.031.0314246
CATGGGG7100.030.4486754
TCAACGC10750.030.1654324
CAACGCA10850.029.8874075
CCCTTAT804.761978E-429.386851
GGGTACC3300.028.4829527
ACTATAC856.8078865E-427.6452143
CTAATAG856.8078865E-427.6452143
ATGGGGC2053.6379788E-1127.5103655
ACGATCA700.0088295126.855359
TATCAAC12150.026.7021262
Produced by FastQC (version 0.11.2)